Isolation and modification of nano-scale cellulose from organosolv-treated birch through the synergistic activity of LPMO and endoglucanases

Nanocellulose isolation from lignocellulose is a tedious and expensive process with high energy harsh chemical requirements, primarily due to the recalcitrance of substrate, which otherwise would have been cost-effective its abundance. Replacing steps biocatalytic processes offers opportunities solve this bottleneck certain extent enzymes substrate specificity mild reaction chemistry. In work, we demonstrate sulphate-free nanocellulose organosolv pretreated birch biomass using different glycosyl-hydrolases, along accessory oxidative including lytic polysaccharide monooxygenase (LPMO). The suggested produced colloidal suspensions (ζ-potential -19.4 mV) particles 7-20 nm diameter, carboxylate content improved thermostability (To = 301 °C, Tmax 337 °C). Nanocelluloses were subjected post-modification LPMOs regioselectivity. sample route was least favorable for LPMO enhance content, while that C1-specific treatment showed highest increase in content.